Gateway™ pT-Rex™-DEST30 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,Leia mais

Número do catálogo	Quantity
12301016 conhecido também como 12301-016	6 μg

Número do catálogo 12301016

conhecido também como 12301-016

Preço (BRL)

Quantity:

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Constitutive or Inducible SystemInducible

Delivery TypeTransfection

For Use With (Application)Regulated Expression

Inducing AgentTetracycline

Product TypeGateway System Destination Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)Neomycin⁄Kanamycin

VectorpDEST

Cloning MethodGateway

Product LineGateway, T-REx

PromoterCMV/TO

Protein TagUntagged

Unit SizeEach

Conteúdo e armazenamento

All destination vectors are provided lyophilized and supercoiled.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pT-Rex™-DEST30 Vector FAQs

Citações e referências (1)

Citações e referências

Abstract

Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells.

Authors:Jones J, Nivitchanyong T, Giblin C, Ciccarone V, Judd D, Gorfien S, Krag SS, Betenbaugh MJ,

Journal:Biotechnol Bioeng

PubMed ID:15981277

The inducible T-REx system and other inducible expression systems have been developed in order to control the expression levels of recombinant protein in mammalian cells. In order to study the effects of heterologous protein expression on mammalian host behavior, the gene for recombinant Human transferrin (hTf) was integrated into HEK-293 ... More