PureLink® Plant RNA Reagent

Catalog number: 12322012

Ambion™  Related applications: RNA Extraction

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Description

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds). Features of the Plant RNA Reagent:

• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol® Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A260/A280 and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5 g of tissue.

Isolated RNA is ideal for a variety of applications and products:

cDNA library construction kits
Real-Time PCR (qPCR)
Northern blotting kits & reagents
Poly(A) selection kit
RNase control reagents & assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Plant Samples
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Scale: Scaleable
Quantity: 1
Product Size: 100 mL
Final Product: Total RNA
Purification Time: 1 hr
Number of Reactions: 200 Preps
Isolation Technology: Organic Extraction
Starting Material (Amount): Up to 1 x 10^7 cells, Up to 1 g tissue

Contents & storage

PureLink® Plant RNA Reagent is supplied as a ready-to-use solution. Store at +4°C.

Documents

Manuals & protocols

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