Cloned AMV First-Strand cDNA Synthesis Kit

Catalog number: 12328032

Invitrogen™  Related applications: Reverse Transcription

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The Cloned AMV First-Strand Synthesis Kit combines the high performance of Invitrogen™ Cloned AMV RT with the convenience of optimized, pre-qualified reagents. The and ! subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen™ Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum® Taq DNA Polymerase for high-specificity PCR or with Platinum® Taq DNA Polymerase High Fidelity for subsequent PCR cloning.
The Cloned AMV First-Strand cDNA Synthesis Kit:

• Uses high-purity Cloned AMV for higher yields
• Includes 5X first-strand buffer with proprietary agents for more robust performance
• Replaces the cDNA Cycle® Kit and AMV Reverse

Transcriptase System
• Detects transcripts in 1.0 pg of RNA (Figure 1)
• Detects various mRNA targets ranging in size from 1.6 kb to 9.4 kb (Figure 2)
For Research Use Only. Not for use in diagnostic procedures.


Reverse Transcriptase: AMV (Recombinant)
Downstream Application: Reverse Transcriptase PCR (RT-PCR)
Reaction Format: Separate Components
Form: Frozen
Format: Tube(s)
Quantity: 375 units
Template: ssRNA
Sensitivity: Low
Product Size: 25 reactions
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice
Number of Reactions: 25 Reactions
Final Product Size(s): 9.4 kb or less
Optimal Reaction Temperature: 45-55° C

Contents & storage

The Cloned AMV First-Strand cDNA Synthesis Kit is supplied with the components listed in Table 1. Store at -70°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols

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