McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
|• High Glucose||• Sodium Pyruvate|
|• L-glutamine|| |
|• Bacto-peptone|| |
|• Phenol Red|| |
The complete formulation
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2
incubator.cGMP Manufacturing and Quality System
Gibco™ McCoy's 5A (modified) is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ McCoy's 5A (modified) product made in our Scotland facility (22300-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum
(FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2
environment to maintain physiological pH.