One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells
One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells
Invitrogen™

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the additionRead more
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Catalog number 12331013
Price (USD)/ 21 x 50 µL/tube
252.65
Online exclusive
275.00 
Save 22.35 (8%)
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Add to cart
Quantity:
21 x 50 μL
Price (USD)/ 21 x 50 µL/tube
252.65
Online exclusive
275.00 
Save 22.35 (8%)
Add to cart
One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells
Catalog number12331013
Price (USD)/ 21 x 50 µL/tube
252.65
Online exclusive
275.00 
Save 22.35 (8%)
-
Add to cart

One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection. The inclusion of the tonA genotype offers added security to protect valuable clones and libraries. MAX Efficiency DH10B T1 cells can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid, making them ideal for generating cDNA or genomic libraries, transforming large plasmids (>150 kb in size), and for situations when DNA is limiting.

MAX Efficiency DH10B T1 Phage-Resistant Cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B-T1R cells an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

MAX Efficiency DH10B T1 Phage-Resistant Cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
mcrA genotypic marker and the mcrBC, mrr deletion that allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation that enables increased plasmid yield and quantity

Convenient and efficient format 
DH10B-T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles, or money wasted on unused cells.

Genotype
FmcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG ton
 
Find the strain and format that fit your needs
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
The DH10B-T1R strain is available in Multishot format for high throughput applications.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChemically Competent Cells
Contains F' EpisomeNo
Improves Plasmid QualityYes (endA1)
Cloning Methylated DNAYes (mcrA)
Transformation Efficiency LevelHigh Efficiency (>1 x 109 cfu/μg)
Antibiotic Resistance BacterialYes (Streptomycin)
Cloning Unstable DNANot suitable for cloning unstable DNA
Blue/White ScreeningYes (lacZΔM15)
High-throughput CompatibilityLow
PlasmidHigh Copy Plasmid
Preparing Unmethylated DNANo
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)Yes
SpeciesE. coli (K12)
FormatTube
Product LineOne Shot™
Quantity21 x 50 μL
Unit Size21 x 50 µL/tube
Contents & Storage
• One Shot MAX Efficiency DH10B T1 Phage-Resistant Cells (21 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (6 ml)
Store S.O.C. Medium at 4°C or room temperature.

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