Invitrogen™

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Catalog number:  12331013

Related applications:

Transformation

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Description

DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B-T1R genotype has the following features:

tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones

DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH10B
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 1x10^9
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Format: One Shot
Improves Plasmid Quality: Yes
Species: E. coli

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).