Invitrogen™

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Catalog number: 12331013
Invitrogen™

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Catalog number: 12331013
Catalog Number
12331013
Unit Size
21 x 50 µL/tube
Price (USD)
248.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1233101321 x 50 µL/tube
248.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B-T1R genotype has the following features:

tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones

DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
DH10B
Blue/White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Product Line
One Shot™
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
T1 Phage - Resistant (tonA)
Yes
Format
One Shot
Product Type
Competent Cells
Species
E. coli
Contains F' Episome
Lacks F' Episome
Efficiency
> 1x10^9
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).

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Frequently asked questions (FAQs)

Citations & References