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Invitrogen™

AccuPrime™ Taq DNA Polymerase System

Catalog number: 12339016
Invitrogen™

AccuPrime™ Taq DNA Polymerase System

Catalog number: 12339016
Catalog Number
12339016
also known as 12339-016
Unit Size
200 rxns
Price (USD)
Price: 421.00
Online price: 383.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 reactions
Price (USD)
Price: 1,960.00
Online price: 1,793.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12339016
also known as 12339-016
200 rxns
Price: 421.00
Online price: 383.65
Your price:
-
123390241,000 reactions
Price: 1,960.00
Online price: 1,793.65
Your price:
-
Product Overview
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The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum™ anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

Advantages of AccuPrime™ Taq DNA Polymerase:
Specificity—control mispriming at every cycle to get single-band PCR products
Ease of use—minimize reaction optimization and primer set redesign
Convenience—conveniently assemble reactions at room temperature

AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
GC-Rich PCR Performance
Low
PCR Method
1-step RT-qPCR, Multiplex qPCR
Polymerase
AccuPrime Taq DNA Polymerase
Reaction Speed
Standard
Product Type
DNA Polymerase System
Shipping Condition
Wet or Dry Ice
For Use With (Application)
Hot-start PCR
Concentration
10X
Fidelity (vs. Taq)
2X
Hot Start
Built-In Hot Start
No. of Reactions
200 Reactions
Overhang
3'-A
Reaction Format
Separate Components
Size (Final Product)
5 kb or less

Contents & Storage

• AccuPrime Taq DNA Polymerase (100 μL, at 2 U/μL)
• 10X AccuPrime PCR Buffer I (500 μL)
• 10X AccuPrime PCR Buffer II (500 μL)
• 50 mM Magnesium Chloride (500 μL)

Store at -20°C.

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