AccuPrime™ Taq DNA Polymerase System
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AccuPrime&trade; <i>Taq</i> DNA Polymerase System
Invitrogen™

AccuPrime™ Taq DNA Polymerase System

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
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Catalog NumberNo. of Reactions
12339016200 Reactions
123390241000 Reactions
Catalog number 12339016
Price (USD)/ 200 rxns
389.65
Online exclusive
427.00 
Save 37.35 (9%)
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Add to cart
No. of Reactions:
200 Reactions
Request bulk or custom format
Price (USD)/ 200 rxns
389.65
Online exclusive
427.00 
Save 37.35 (9%)
Add to cart
AccuPrime™ Taq DNA Polymerase System
Catalog number12339016
Price (USD)/ 200 rxns
389.65
Online exclusive
427.00 
Save 37.35 (9%)
-
Add to cart
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

AccuPrime Taq DNA Polymerase features

  • Control mispriming at every cycle for single-band PCR products
  • Minimal reaction optimization and primer set redesign
  • Reaction assembly at room temperature

Applications

AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:

  • Multiplex PCR
  • TOPO TA Cloning
  • Allele-specific amplifications
  • High-throughput screening

AccuPrime buffers

The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PCR Method1-step RT-qPCR, Multiplex qPCR
PolymeraseAccuPrime Taq DNA Polymerase
Reaction SpeedStandard
Product TypeDNA Polymerase System
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Concentration10X
Fidelity (vs. Taq)2X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Unit Size200 rxns
Contents & Storage
• AccuPrime Taq DNA Polymerase (100 μL, at 2 U/μL)
• 10X AccuPrime PCR Buffer I (500 μL)
• 10X AccuPrime PCR Buffer II (500 μL)
• 50 mM Magnesium Chloride (500 μL)

Store at -20°C.