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Invitrogen™

AccuPrime™ Taq DNA Polymerase System

Catalog number: 12339016
Invitrogen™

AccuPrime™ Taq DNA Polymerase System

Catalog number: 12339016
Catalog Number
12339016
also known as 12339-016
Unit Size
200 reactions
Price (USD)
349.00
Your Price:
Availability
***
Quantity
Catalog Number
12339024
also known as 12339-024
Unit Size
1,000 reactions
Price (USD)
1,624.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12339016
also known as 12339-016
200 reactions
349.00
Your Price:
***
12339024
also known as 12339-024
1,000 reactions
1,624.00
Your Price:
***
Product Overview
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The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. Advantages of AccuPrime™ Taq DNA Polymerase:

Specificity—control mispriming at every cycle to get single-band PCR products
Ease of use—minimize reaction optimization and primer set redesign
Convenience—conveniently assemble reactions at room temperature

AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning®, and allele-specific amplifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity
5' - 3'
For Use With (Application)
PCR Amplification
No. of Tests
200 Reactions
Product Line
AccuPrime™
Product Type
DNA Polymerase System
Shipping Condition
Approved for shipment on Wet or Dry Ice
Sufficient For
200 Reactions
Concentration
10 X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
Low
Hot Start
Built-In Hot Start
No. of Reactions
200
Overhang
3'-A
PCR Method
Multiplex PCR, RT-PCR
Polymerase
AccuPrime™ Taq DNA Polymerase
Reaction Format
Separate Components
Reaction Speed
Standard
Size (Final Product)
5 kb or less

Contents & Storage

AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes:

• AccuPrime™ Taq DNA Polymerase (100 µL, at 2U/µL)

• 10X AccuPrime™ PCR Buffer I* (500 µL)

• 10X AccuPrime™ PCR Buffer II* (500 µL)

• 50 mM Magnesium Chloride (500 µL)

*10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.

Store at -20°C.

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Frequently asked questions (FAQs)

Citations & References