AccuPrime™ Taq DNA Polymerase System

Catalog number: 12339024

Invitrogen™  Related applications: Methylation Analysis | PCR

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Description

The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. Advantages of AccuPrime™ Taq DNA Polymerase:

Specificity—control mispriming at every cycle to get single-band PCR products
Ease of use—minimize reaction optimization and primer set redesign
Convenience—conveniently assemble reactions at room temperature

AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning®, and allele-specific amplifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 1,000 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

AccuPrime™ Taq DNA Polymerase is provided in a 1000 reaction kit. Each kit includes:

• AccuPrime™ Taq DNA Polymerase (500 µL, at 2U/µL)

• 10X AccuPrime™ PCR Buffer I* (2 × 1.25 mL)

• 10X AccuPrime™ PCR Buffer II* (2 × 1.25 mL)

• 50 mM Magnesium Chloride (500 µL)

*10XAccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.

Store at -20°C.

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