This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.
Get out of the cold & warm up to new Gibco BenchStable Media! Optimized for storage at room temperature »
Gibco™

Medium 199, HEPES This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  12340030

Related applications:

Mammalian Cell Culture

Back to top

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:

With
• Phenol Red
• HEPES
• L-glutamine

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22340-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Glucose: Low Glucose
Green Features: Sustainable packaging
HEPES Buffer: HEPES
Product Line: Gibco®
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml
Form: Liquid
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations