AccuPrime™ SuperMix II

Catalog number: 12341020

Invitrogen™  Related applications: Methylation Analysis | PCR

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Description

AccuPrime™ SuperMix II is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).

For accurate PCR performance, AccuPrime™ SuperMix II sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime™ SuperMix I includes Platinum® anti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.

AccuPrime™ SuperMix II is designed for amplification of genomic DNA amplicons, plasmid DNA, or cDNA templates up to 4kb in length. Alternatively, AccuPrime™ SuperMix I is optimized to amplify a tighter range of targets less than 200bp in length.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 4 kb or less
Exonuclease Activity: 5' - 3'
Product Size: 1,000 reactions
Shipping Condition: Dry Ice

Contents & storage

AccuPrime™ SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 1,000 amplification reactions of 25 ?l each are provided.

AccuPrime™ SuperMix II Components:
40 mM Tris-HCl (pH 8.4); 100 mM KCl; 3 mM MgCl2, 400 ?M each dGTP, dATP, dTTP, dCTP; AccuPrime™ Taq DNA Polymerase; thermostable AccuPrime™ protein; stabilizers.

1,000 rxns: 1 × 12.5 ml

Sore at -20°C in a non-frost-free freezer.

Documents

Manuals & protocols