AccuPrime™ Pfx DNA Polymerase

Catalog number: 12344024

Invitrogen™  Related applications: Methylation Analysis | PCR

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone. With AccuPrime™ Pfx DNA Polymerase you will:

• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimizationApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ Pfx DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 26 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 12 kb or less
Product Size: 200 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

AccuPrime™ Pfx DNA Polymerase (100 µL, at 2.5U/µL) is provided with 10X AccuPrime™ reaction mix (1 mL) that contains dNTPs and a separate vial (1 mL) of 50 mM MgSO4.

Reagents are provided for 200 amplification reactions of 50 µl each. Store at -20°C.

Documents

Manuals & protocols

Recommended products