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Invitrogen™
AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
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Catalog Number
No. of Reactions
12344032
1000 Reactions
12344024
200 Reactions
Catalog number 12344032
Price (USD)/ 1,000 reactions
2,463.65
Online exclusive
2585.00
Save 121.35 (5%)
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Add to cart
No. of Reactions:
1000 Reactions
Request bulk or custom format
Price (USD)/ 1,000 reactions
2,463.65
Online exclusive
2585.00
Save 121.35 (5%)
Add to cart
AccuPrime™ Pfx DNA Polymerase
Catalog number12344032
Price (USD)/ 1,000 reactions
2,463.65
Online exclusive
2585.00
Save 121.35 (5%)
-
Add to cart
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
Greater than 26 times higher fidelity than Taq DNA polymerase
Amplification of fragments up to 12 kb
Minimized PCR optimization
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates
RT-PCR
High-fidelity PCR
Hot-start PCR
Notes
AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PolymeraseAccuPrime Pfx DNA Polymerase
Reaction SpeedStandard
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)26X
Hot StartBuilt-In Hot Start
No. of Reactions1000 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)12 kb or less
Volume500 μL
Unit Size1,000 reactions
Contents & Storage
• AccuPrime Pfx DNA Polymerase (1 x 500 μL at 2.5 U/μL) • 10X AccuPrime Pfx Reaction Mix (5 x 1 mL) • 50 mM MgSO4 (2 x 1 mL)