AccuPrime™ Pfx SuperMix

Catalog number: 12344040

Invitrogen™  Related applications: Methylation Analysis | PCR

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Description

AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. With AccuPrime™ Pfx SuperMix you will:

• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Produce products with blunt ends

Using AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ Pfx DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 26 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 15 kb or less
Product Size: 200 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

AccuPrime™ Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 µl each (4 × 1.125 ml) are provided.

AccuPrime™ Pfx SuperMix Components: 22 U/ml Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66 mM Tris-SO4 (pH 8.4), 30.8 mM (NH4)2SO4, 11 mM KCl, 1.1 mM MgSO4, 330 µM dNTPs, AccuPrime™ proteins, stabilizers.

Store at -20°C in a non-frost-free freezer.

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