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Invitrogen™

AccuPrime™ Pfx SuperMix

Catalog number: 12344040
Invitrogen™

AccuPrime™ Pfx SuperMix

Catalog number: 12344040
Catalog Number
12344040
also known as 12344-040
Unit Size
200 rxns
Price (USD)
Price: 714.00
Online price: 652.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12344040
also known as 12344-040
200 rxns
Price: 714.00
Online price: 652.65
Your price:
-
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AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half.

With AccuPrime™ Pfx SuperMix you will:
• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Produce products with blunt ends

Using AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
GC-Rich PCR Performance
Low
Polymerase
AccuPrime Pfx DNA Polymerase
Reaction Speed
Standard
Shipping Condition
Wet or Dry Ice
For Use With (Application)
Hot-start PCR, High-fidelity PCR
Concentration
1.1X
Fidelity (vs. Taq)
26X
Hot Start
Built-In Hot Start
No. of Reactions
200 Reactions
Overhang
Blunt
Reaction Format
SuperMix or Master Mix
Size (Final Product)
15 kb or less

Contents & Storage

AccuPrime Pfx SuperMix (4 × 1.125 mL)

Store at –20°C in a non-frost-free freezer.

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