Gibco™

      293fectin™ Transfection Reagent

      293fectin™ Transfection Reagent

      Catalog number:  12347019

       Related applications: Antibody & Cell Culture Production | Protein Expression | Transfection

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      Description

      293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

      • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
      • Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
      • Add 293fectin™ reagent complexes directly to cells in culture medium
      • No need to remove complexes or change or add medium following transfection
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Cell Type: Established Cell Lines
      Sample Type (Specific): Plasmid DNA
      Transfection Technique: Lipid-Based Transfection
      Serum Compatible: Yes
      High Throughput Compatibility: Not High Throughput-Compatible (Manual)
      Product Line: 293fectin™
      Product Size: 1 mL

      Contents & storage

      Each unit contains one vial of reagent. Store at +4°C. 293fectin™ is guaranteed stable for 6 months when stored properly.

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