Gibco™

293fectin™ Transfection Reagent

Catalog number:  12347019

Related applications:

Antibody & Cell Culture Production

| Protein Expression

| Transfection

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Description

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Established Cell Lines
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Line: 293fectin™
Product Size: 1 mL
Sample Type (Specific): Plasmid DNA
Serum Compatible: Yes
Transfection Technique: Lipid-Based Transfection

Contents & storage

Each unit contains one vial of reagent. Store at +4°C. 293fectin™ is guaranteed stable for 6 months when stored properly.