Invitrogen™

Platinum™ SuperFi™ DNA Polymerase

Catalog number: 12351010
Invitrogen™

Platinum™ SuperFi™ DNA Polymerase

Catalog number: 12351010
Catalog Number
12351010
also known as 12351-010
Unit Size
100 units
Price (USD)
222.00
Your Price:
Availability
***
Quantity
Catalog Number
12351050
also known as 12351-050
Unit Size
500 units
Price (USD)
1,018.00
Your Price:
Availability
***
Quantity
Catalog Number
12351250
also known as 12351-250
Unit Size
5 x 500 units
Price (USD)
4,280.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12351010
also known as 12351-010
100 units
222.00
Your Price:
***
12351050
also known as 12351-050
500 units
1,018.00
Your Price:
***
12351250
also known as 12351-250
5 x 500 units
4,280.00
Your Price:
***
Product Overview
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Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Benefits of Platinum SuperFi DNA Polymerase include:
• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
• Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Applications of Platinum SuperFi DNA Polymerase

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Using Platinum SuperFi DNA Polymerase
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

Platinum SuperFi DNA Polymerase is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

More Platinum SuperFi DNA Polymerase products ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq)
300 X
Hot Start
Built-In Hot Start
Product Line
Platinum™, SuperFi™
Shipping Condition
Approved for shipment on Wet or Dry Ice
GC-Rich PCR Performance
High
Overhang
Blunt
Polymerase
Platinum SuperFi DNA Polymerase
Quantity
100 Unit(s)
Reaction Format
Separate Components
Reaction Speed
Fast

Contents & Storage

• 50 µL Platinum SuperFi DNA Polymerase (2U/µL)
• 2 x 1.25 mL SuperFi Buffer (5X)
• 1.25 mL SuperFi GC Enhancer (5X)

Store at -5 to -30°C.

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Frequently asked questions (FAQs)

Citations & References