Pfx50™ DNA Polymerase

Catalog number: 12355036

Invitrogen™  Related applications: PCR

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Description

Pfx50™ DNA Polymerase is a designer enzyme derived from the archaean Thermococcus zilligii. In addition to 3´ to 5´ proofreading ability, Pfx50 polymerase is fused to an accessory protein for enhanced fidelity and processivity. This unique combination makes the Pfx50 enzyme ideal for applications that require the highest fidelity and PCR success the first time. With Pfx50 DNA Polymerase, you will:

• Achieve ultra low error rates—50 times better than Taq, without compromising PCR robustness
• Save time and money—highest fidelity means youll sequence fewer clones to obtain error-free constructs
• Produce results quickly and easily—robust performance with extremely short extension holds

Downstream compatibility with popular cloning techniques
Pfx50 DNA Polymerase produces blunt-ended PCR products for compatibility with Directional TOPO® Expression Kits and Zero Blunt® TOPO® PCR Cloning Kits.

Unit Definition:
One unit of Pfx50 DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min at 74°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Pfx50™ DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 50 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 4 kb or less
Product Size: 500 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Dry Ice

Contents & storage

Pfx50 DNA Polymerase is supplied with a vial of 10X Pfx50 PCR amplification buffer contains 1.2 mM MgSO4 and a vial of 50 mM MgSO4 for further optimization. Store at -20°C. Guaranteed stable for 6 months when properly stored.

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