Invitrogen™ SuperFi™ Green Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction. SuperFi Green Buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with the enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless SuperFi™ Buffer .