Platinum™ SuperFi™ PCR Master Mix

Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Features

• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult targets of high GC content
• Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
• Amplification of samples with suboptimal purity

Applications

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Long PCR

Notes

• Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
• Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).