Invitrogen™

Platinum™ SuperFi II PCR Master Mix

Catalog number: 12368010
Invitrogen™

Platinum™ SuperFi II PCR Master Mix

Catalog number: 12368010
Catalog Number
12368010
Unit Size
100 reactions
Price (USD)
300.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
1,366.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 500 reactions
Price (USD)
6,110.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12368010100 reactions
300.00
Your Price:
***
12368050500 reactions
1,366.00
Your Price:
***
123682505 x 500 reactions
6,110.00
Your Price:
***
Product Overview
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Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification. Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Features of Platinum SuperFi II PCR Master Mix include:
• Exceptional >300X Taq fidelity
• Universal primer annealing at 60°C
• Superior specificity, sensitivity, and yields
• Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications of Platinum SuperFi II DNA Polymerase:
• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Platinum SuperFi II Green PCR Master Mix is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis.

View more information about Platinum SuperFi II DNA Polymerase products ›

Specifications

Fidelity (vs. Taq)
>300X
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
Overhang
Blunt
Polymerase
Platinum SuperFi II DNA Polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Product Line
Platinum™, SuperFi™
Product Type
PCR Master Mix
Shipping Condition
Dry Ice

Contents & Storage

• 2 x 1.25 mL Platinum SuperFi II PCR Master Mix
• 2 x 1.25 mL water, nuclease-free

Sufficient for 100 50-µL reactions

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