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Catalog Number | Color | No. of Reactions |
---|---|---|
12369050 | Green | 500 Reactions |
12369010 | Green | 100 Reactions |
12369250 | Green | 5 x 500 Reactions |
12368010 | Colorless | 100 Reactions |
12368050 | Colorless | 500 Reactions |
12368250 | Colorless | 5 x 500 Reactions |
Features of Platinum SuperFi II PCR Master Mix include:
Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction set up at room temperature and provides increased sensitivity and yield.
Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.
Applications of Platinum SuperFi II DNA Polymerase:
Platinum SuperFi II PCR Master Mix (12368010) is also available, which is the same master mix without the tracking dyes.