CD Hybridoma AGT™ Medium

Catalog number: 12372001

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture

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Description

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual.

Product Use
For Research Use Only or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Powder (AGT)
Glucose: Low Glucose
Cell Type: Hybridomas, Myeloma Cells
Glutamine: No Glutamine
Sterility: Nonsterile
Serum Level: Serum-Free
Concentrated: 1 X
HEPES Buffer: No HEPES
Product Size: 100 L
Classification: Animal Origin-Free, Protein-Free, Chemically Defined
Endotoxin Level: Low
Inorganic Salts: Sodium Phosphate, Calcium, Magnesium
Culture Environment: CO2
Included Antibiotics: No Antibiotics
Phenol Red Indicator: No Phenol Red
Purity or Quality Grade: Certified
Sodium Pyruvate Additive: Sodium Pyruvate
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Shipping Condition: Wet Ice

Contents & storage

Storage conditions: 2-8°C, dark and dry
Shipping conditions: Gel packs
Shelf life: 24 months from date of manufacture

Documents

Manuals & protocols

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