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Gibco™
DMEM/F-12, powder, HEPES
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12400024 | 10 x 1 L |
카탈로그 번호 12400024
제품 가격(KRW)
160,000
온라인 행사
Ends: 31-Dec-2025
168,000할인액 8,000 (5%)
Each
수량:
10 x 1 L
제품 가격(KRW)
160,000
온라인 행사
Ends: 31-Dec-2025
168,000할인액 8,000 (5%)
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
This DMEM/F-12 is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • HEPES | |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
사양
세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12
수량10 x 1 L
유통 기한36 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Powder
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
첨가제 없음No Sodium Bicarbonate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture