Gibco™

DMEM/F-12, powder, HEPES

Catalog number: 12400024
Gibco™

DMEM/F-12, powder, HEPES

Catalog number: 12400024
Catalog Number
12400024
Unit Size
10 x 1 L
Price (USD)
Price: 103.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1240002410 x 1 L
Price: 103.00
Your Price:
-
Product Overview
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Additional Information
Recommendations
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
WithWithout
• L-glutamine• Sodium Bicarbonate
• HEPES
• Phenol Red

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Product Type
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Product Line
Gibco™
Form
Powder
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
36 Months From Date of Manufacture
With Additives
L-Glutamine, Phenol Red, HEPES
Without Additives
Sodium Bicarbonate
Glucose
High Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
Cell Line
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)/F-12
HEPES Buffer
HEPES

Contents & Storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture