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Invitrogen™

pcDNA™3.2-DEST Mammalian Expression Vector

Catalog number:  12489019

Related applications:

Antibody & Cell Culture Production

| Gateway Cloning

| Protein Expression

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Description

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Gateway™, pcDNA™
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
Promoter: CMV
Product Type: Mammalian Expression Vector
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Key Functions: Constitutive Expression
Protein Tag: V5 Epitope Tag
Cloning Method: Gateway™
Vector: pDEST, pcDNA

Contents & storage

All destination vectors are provided lyophilized and supercoiled.