pcDNA™3.2-DEST Mammalian Expression Vector

Catalog number: 12489019

Invitrogen™  Related applications: Antibody & Cell Culture Production | Gateway Cloning | Protein Expression

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Description

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pcDNA, pDEST
Product Size: 6 µg
Constitutive or Inducible System: Constitutive

Contents & storage

All destination vectors are provided lyophilized and supercoiled.

Documents

Manuals & protocols

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