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Invitrogen™

pcDNA™3.2-DEST Mammalian Expression Vector

Catalog number: 12489019
Invitrogen™

pcDNA™3.2-DEST Mammalian Expression Vector

Catalog number: 12489019
Catalog Number
12489019
also known as 12489-019
Unit Size
6 µg
Price (USD)
Price: 756.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12489019
also known as 12489-019
6 µg
Price: 756.00
Your Price:
-
Product Overview
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Additional Information
Recommendations
Recommendations
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
CMV
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
V5 Epitope Tag
Cloning Method
Gateway™
Vector
pDEST, pcDNA
Product Line
Gateway™, pcDNA™
For Use With (Application)
Constitutive Expression

Contents & Storage

All destination vectors are provided lyophilized and supercoiled.