CD CHO AGT™ Medium

Catalog number: 12490003

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture

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Description

CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.

When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: CHO
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Culture Environment: CO2
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Form: Powder (AGT)
Format: Ropak Bucket
Glucose: High Glucose
Glutamine: No Glutamine
HEPES Buffer: No HEPES
Included Antibiotics: No antibiotics
Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
Phenol Red Indicator: No Phenol Red
Product Size: 10 kg
Purity or Quality Grade: Certified
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Sterility: Nonsterile
Supplements: No Hypoxanthine, No Thymidine
Shipping Condition: Dry Ice

Contents & storage

Store at 2 to 8°C in the dark.

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Manuals & protocols

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