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Gibco™

Advanced DMEM

Catalog number: 12491015
Have Questions?
Gibco™

Advanced DMEM

Catalog number: 12491015
Have Questions?
Catalog Number
12491015
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
10 x 500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12491015500 mL
-
1249102310 x 500 mL
-
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Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.


This Advanced DMEM is manufactured as follows:
WithWithout
• High glucose• L-glutamine
• Non Essential Amino Acids 
• Sodium pyruvate 
• Phenol Red 


The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line
MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Classification
Animal Origin
Concentration
1 X
Form
Liquid
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Serum Level
Reduced Serum
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives
No Glutamine, No HEPES
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

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