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Gibco™

Advanced DMEM

Green features
Catalog number: 12491015
Gibco™

Advanced DMEM

Green features
Catalog number: 12491015
Catalog Number
12491015
also known as 12491-015
Unit Size
500 mL
Price (USD)
41.07
Your Price:
Availability
***
Quantity
Catalog Number
12491023
also known as 12491-023
Unit Size
10 x 500 mL
Price (USD)
354.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12491015
also known as 12491-015
500 mL
41.07
Your Price:
***
12491023
also known as 12491-023
10 x 500 mL
354.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:
WithWithout
• High glucose• L-glutamine
• Non Essential Amino Acids
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Advanced DMEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Serum Supplementation
Advanced (Reduced Serum)
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
No Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Quantity
500mL
With Additives
High Glucose, Non Essential Amino Acids, Phenol Red, Sodium Pyruvate
Without Additives
L-Glutamine
Glucose
High Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
Cell Line
MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Classification
Animal Origin
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

Figures