Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Using DMEM/F-12
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ DMEM / F-12 product made in our Scotland facility (32500-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.