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Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Catalog number:  12532024

Related applications:

PCR

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Description

Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:

• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 3' - 5', 5' - 3'
Fidelity (vs. Taq): 6 X
GC-Rich PCR Performance: Low
Hot Start: Built-In Hot Start
Overhang: Mixed
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Product Line: Platinum®
Quantity: 5000 reactions
Reaction Format: SuperMix or Master Mix
Reaction Speed: Standard
Shipping Condition: Approved for shipment on Wet or Dry Ice
Size (Final Product): 15 kb or less

Contents & storage

• 4 × 56.25 mL Platinum PCR SuperMix High Fidelity

Store at -20°C in a non-frost-free freezer.