Platinum™ PCR SuperMix High Fidelity

Description

Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:

• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

Figures

Specifications

Polymerase:	Platinum® Taq DNA Polymerase High Fidelity
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	6 X
GC-Rich PCR Performance:	Low
Reaction Format:	SuperMix or Master Mix
Product Overhang:	Mixed
Reaction Speed:	Standard
Final Product Size(s):	15 kb or less
Exonuclease Activity:	3' - 5', 5' - 3'
Product Line:	Platinum®
Product Size:	5,000 reactions
Shipping Condition:	Approved for shipment on Wet or Dry Ice

Contents & storage