Upgrade to Invitrogen™ Platinum™ SuperFi™ DNA Polymerase: >100-fold higher fidelity than Taq, robust and versatile.
Invitrogen™
Platinum™ PCR SuperMix High Fidelity
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Description
Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization
Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Polymerase: | Platinum® Taq DNA Polymerase High Fidelity |
|---|---|
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 6 X |
| GC-Rich PCR Performance: | Low |
| Reaction Format: | SuperMix or Master Mix |
| Product Overhang: | Mixed |
| Reaction Speed: | Standard |
| Final Product Size(s): | 15 kb or less |
| Exonuclease Activity: | 3' - 5', 5' - 3' |
| Product Line: | Platinum® |
| Product Size: | 5,000 reactions |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
Contents & storage
Platinum® PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000 amplification reactions of 50 ?l each are provided.
Platinum® PCR SuperMix High Fidelity Components:
22 U/ml complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum® Taq Antibody; 66 mM Tris-SO4 (pH 8.9); 19.8 mM (NH4)2SO4; 2.4 mM MgSO4; 220 ?M dNTPs; and stabilizers.
5,000 rxns: 4 × 56.25 ml
Sore at -20°C in a non-frost-free freezer.
Platinum® PCR SuperMix High Fidelity Components:
22 U/ml complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum® Taq Antibody; 66 mM Tris-SO4 (pH 8.9); 19.8 mM (NH4)2SO4; 2.4 mM MgSO4; 220 ?M dNTPs; and stabilizers.
5,000 rxns: 4 × 56.25 ml
Sore at -20°C in a non-frost-free freezer.
