PCR Cloning System with Gateway® Technology with pDONR™221 & OmniMAX™2 Competent Cells

Catalog number: 12535029

Invitrogen™  Related applications: Gateway Cloning

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Description

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:

• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: pDONR
Cloning Method: Gateway®
Product Size: 20 reactions

Contents & storage

The PCR Cloning Kit with Gateway® Technology includes Gateway® BP Clonase® II enzyme mix and reagents, Gateway® pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot® OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.

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