Invitrogen™

PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Catalog number: 12535037
Invitrogen™

PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Catalog number: 12535037
Catalog Number
12535037
also known as 12535-037
Unit Size
20 reactions
Price (USD)
Price: 716.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12535037
also known as 12535-037
20 reactions
Price: 716.00
Your price:
-
Product Overview
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Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway™ Technology. The PCR Cloning System with Gateway™ Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway™ Technology offers:

• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method
Gateway™
Product Line
Gateway™, OmniMAX™, pDONR™
Product Type
PCR Cloning System
Vector
pDONR

Contents & Storage

The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reaction buffer, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.

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