The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector offers the following key features:
•Promoterless version of the pcDNA™6.2⁄V5-DEST vector (cat. no. 12489027) •attR sites for Gateway™ cloning •Compatible with MultiSite Gateway™ Pro kits (e.g. cat. no. 12537100) •C-terminal V5 tag for easy detection •Blasticidin resistance gene for efficient stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Delivery Type
Transfection
For Use With (Equipment)
MultiSite Gateway™ Pro kits (e.g. cat. no. 12537-100)
Promoter
None (Promoterless)
Product Type
Mammalian Expression Vector
Expression System
Mammalian
Protein Tag Position (to your gene)
C-terminal
Protein Tag
V5 Epitope Tag
Cloning Method
Gateway
Vector
pcDNA, pDEST, MultiSite Gateway Vectors
Product Line
Gateway™, pcDNA™
For Use With (Application)
Clone your own Promoter, Multi-Gene Expression
Contents & Storage
All destination vectors are provided lyophilized and supercoiled.