This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.
Gibco™

William's E Medium, no glutamine

Green features
Catalog number: 12551032
Gibco™

William's E Medium, no glutamine

Green features
Catalog number: 12551032
Catalog Number
12551032
also known as 12551-032
Unit Size
500 mL
Price (USD)
44.50
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12551032
also known as 12551-032
500 mL
44.50
Your Price:
***
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

For Use With (Application)
Hepatocytes and hepatic models, mammalian cell culture, pharma and biopharma, primary cell culture
Product Type
William's E Medium
HEPES Buffer
No HEPES
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
No Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months
Green Features
Sustainable packaging
Quantity
500mL
Without Additives
Sodium Pyruvate
Culture Type
Mammalian Cell Culture
Sodium Pyruvate Additive
Sodium Pyruvate

Contents & Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

Figures

Frequently asked questions (FAQs)

Citations & References