Gibco™

TrypLE™ Select Enzyme (1X), no phenol red

Catalog number: 12563011
Have Questions?
Gibco™

TrypLE™ Select Enzyme (1X), no phenol red

Catalog number: 12563011
Have Questions?
Catalog Number
12563011
Unit Size
100 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12563011100 mL
-
12563029500 mL
-
Product Overview
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TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols, with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

>This TrypLE™ Select is modified as follows:

With
• EDTA

Without
• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Chelators
EDTA
Cell Type
Mammalian Cells
Classification
Animal Origin-free
Concentration
1 X
Culture Type
Adherent Cell Culture
Form
Liquid
Product Type
Cell Culture Dissociation Reagent
Shelf Life
24 Months
Without Additives
No Phenol Red
Endotoxin Level
Low
Format
Bottle(s)
Green Features
Energy efficient, Sustainable packaging
Osmolality
280 - 300 mOsm/kg
pH
7 to 7.4
Product Line
Gibco™
Quantity
100 mL
Remarks
Room Temperature Stable
Shipping Condition
Room Temperature
Source
Animal origin-free
Tested For
In vitro Bioassay

Contents & Storage

Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.

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