Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
AuthorsCheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K
JournalJ Mol Cell Biol
PubMed ID21228011
'If induced pluripotent stem (iPS) cells are to be used to treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPS cells from their tissues. To determine the feasibility of using this technology with elderly patients, we asked if it was indeed possible to ... More
A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.
AuthorsShi S, Condon RG, Deng L, Saunders J, Hung F, Tsao YS, Liu Z
JournalJ Vis Exp
PubMed ID21968840
'The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated ... More
Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.
The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney ... More
Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human Fcgamma RIII and Antibody-dependent Cellular Toxicity.
Authors Shields Robert L; Lai Jadine; Keck Rodney; O'Connell Lori Y; Hong Kyu; Meng Y Gloria; Weikert Stefanie H A; Presta Leonard G;
JournalJ Biol Chem
PubMed ID11986321
Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that were deficient in fucose attached to the Asn(297)-linked carbohydrate but were otherwise similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from human serum. Lack of fucose on ... More
Beta -catenin is essential and sufficient for skeletal myogenesis in p19 cells.
Authors Petropoulos Helen; Skerjanc Ilona S;
JournalJ Biol Chem
PubMed ID11856745
Wnt1 and Wnt3a are signaling factors known to play a role in the induction of myogenesis in the myotome of the differentiating somite. Both factors may transduce their signal by a conserved pathway that leads to transcriptional regulation by beta-catenin/Lef1. beta-Catenin and Lef1 are found in the myotome prior to ... More
Mechanisms of FOXC2- and FOXD1-mediated regulation of the RI alpha subunit of cAMP-dependent protein kinase include release of transcriptional repression and activation by protein kinase B alpha and cAMP.
Pathways of induction of peroxiredoxin I expression in osteoblasts: roles of p38 mitogen-activated protein kinase and protein kinase C.
Authors Li Baojie; Ishii Tetsuro; Tan Choon Ping; Soh Jae-Won; Goff Stephen P;
JournalJ Biol Chem
PubMed ID11796722
Peroxiredoxin I (Prx I) is an oxidative stress-inducible antioxidant protein with thioredoxin peroxidase activity. Here we report that the levels of Prx I mRNA and protein are dramatically increased in a murine osteoblast cell line, MC3T3-E1, by treatment with sodium arsenate. We further studied the signaling pathways that control the ... More
Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization.
Osteoblasts mineralize bone matrix by promoting hydroxyapatite crystal formation and growth in the interior of membrane-limited matrix vesicles (MVs) and by propagating the crystals onto the collagenous extracellular matrix. Two osteoblast proteins, tissue-nonspecific alkaline phosphatase (TNAP) and plasma cell membrane glycoprotein-1 (PC-1) are involved in this process. Mutations in the ... More
Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.
Authors Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;
JournalJ Biol Chem
PubMed ID7493973
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More