MEM α, nucleosides
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Gibco™
MEM α, nucleosides
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negativeRead more
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Catalog NumberQuantity
1257107110 x 500 mL
12571063500 mL
125710481000 mL
125710896 x 1000 mL
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Catalog number 12571071
Price (USD)/ 10 x 500 mL
477.65
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500.00 
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10 x 500 mL
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Price (USD)/ 10 x 500 mL
477.65
Online exclusive
500.00 
Save 22.35 (4%)
Add to cart
MEM α, nucleosides
Catalog number12571071
Price (USD)/ 10 x 500 mL
477.65
Online exclusive
500.00 
Save 22.35 (4%)
-
Add to cart

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM α is modified as follows:
With
• Ribonucleosides
• Deoxyribonucleosides
• Phenol Red
• L-glutamine


The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeMEM α (Minimum Essential Medium α)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without AdditivesNo HEPES
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 x 500 mL
Unit Size10 x 500 mL
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture