The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA. Key advantages of this kit:
• Sensitive—routine detection down to 0.01 pg total RNA (see figure) • Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability • Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure) • Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
SuperScript® III Reverse Transcriptase SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
Platinum® Taq DNA polymerase Platinum® Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Application)
200μL SuperScript III RT/Platinum Taq Mix; 3 × 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 3.2mM MgSO4); 500μL 5mM Magnesium Sulfate
No. of Tests
Primer Probe Compatibility
One-Step RT-PCR System
GC-Rich PCR Performance
Built-In Hot Start
No. of Reactions
Platinum™ Taq DNA Polymerase
Size (Final Product)
4.5 kb or less
Contents & Storage
Components: • 200 µL SuperScript™ III RT/Platinum™ Taq Mix • 3 × 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 3.2 mM MgSO4) • 500 µL 5 mM Magnesium Sulfate
Store all components at -30°C to -10°C.
The SuperScript® One-Step RT-PCR System with Platinum® <i>Taq</i> DNA Polymerase.
The SuperScript® III One-Step RT-PCR System with Platinum® <i>Taq</i> DNA Polymerase.
The SuperScript® III One-Step RT-PCR System with Platinum® <i>Taq</i> provides the highest sensitivity available.