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Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574026
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574026
Catalog Number
12574026
Unit Size
100 rxns
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
25 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12574026100 rxns
-
1257401825 rxns
-
Product Overview
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The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 μg of total RNA.

Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
• Sensitive detection down to 0.01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript III Reverse Transcriptase
SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.

Note: This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Gel Electrophoresis
Format
One-Step RT-PCR System
GC-Rich PCR Performance
High
PCR Method
1-step RT-PCR
Polymerase
Platinum Taq DNA Polymerase
Reaction Speed
30 min.
Technique
1-Step RT-PCR
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Final Product Type
PCR Amplified cDNA
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
Reaction Format
Premixed Components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 4.5 kb
Starting Material
RNA

Contents & Storage

• SuperScript III RT/Platinum Taq Mix (200 μL)
• 2X Reaction Mix (3 x 1 mL)
• 5 mM Magnesium Sulfate (500 μL)

Store at –10 to –30°C.

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