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View additional product information for AcTEV™ Protease - FAQs (12575015, 12575023)
3 product FAQs found
A final concentration of 1 mM DTT is required for the AcTEV protease reaction. The DTT serves as a stabilizer of secondary structure, i.e., it ensures that the enzyme does not undergo oxidation. If you are unable to use DTT due to a column purification after digestion, you can leave it out and still see a successful AcTEV digestion, however we recommend using it.
No rigorous quantitative data for activity in the absence of DTT and EDTA has been collected in-house. Incubations using reaction buffer +/-DTT and +/- EDTA; have been performed, but the products were analyzed only at t=0 and t=2 hr at 30 degrees C. No difference in the amount of product generated +/-DTT or +/-EDTA was noticed, but it is possible that the detailed kinetics in a time course reaction may be a little different.
The recognition site for AcTEV Protease is ENLYFQS/G
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
We offer the following products:
-AcTEV Protease (Cat. Nos. 12575015, 12575023)
-EKMax Enterokinase (Cat. Nos. E18001, E18002)
-SUMO Protease (Cat. No. 12588018)
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.