Invitrogen™

Anchored Oligo(dT)20 Primer

Catalog number:  12577011

Related applications:

Reverse Transcription

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Description

Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC). Due to the variable end-sequence, the primer is anchored to the 5´-end of the poly(A) tail of mRNA and prevents priming within the poly(A) tail. Anchored Oligo(dT)20 Primer is recommended for use in cDNA labeling protocols and in RT-PCR.

Quality Control: This product is qualified in a first-strand cDNA synthesis reaction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Primer: Oligo dT Primers
5'Primer Modification: Phosphate
Internal Modification: A+C+G+T, A+C+G
Mass: 50 µg
Primer Length: 22 -mer
Primer Sequence: 5'd PO4 [(T)20VN]3'
Purification Method: Gel-Purified, Desalted
Shipping Condition: Dry Ice

Contents & storage

Anchored Oligo(dT)20 Primer is supplied at 2.5 µg/µl in DEPC-treated water. Store at -20°C. Guaranteed stable for 6 months when properly stored.