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Platinum® Blue PCR SuperMix

Catalog number: 12580015

Invitrogen™  Related applications: PCR

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Platinum® Blue PCR SuperMix provides qualified reagents for the amplification of nucleic acid templates by PCR, and includes blue tracking dye for subsequent gel analysis. The reagents are premixed for high-throughput applications as well as general ease of use.

Using Platinum® Blue PCR SuperMix
The mixture contains recombinant Platinum® Taq DNA polymerase, Mg++, dNTPs, glycerol (used as a precipitant), and blue tracking dye. Platinum® Blue PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided sufficient for 100 or 1,000 amplification reactions of ~50 µL each.

Hot-start specificity
Specific binding of the anti-Taq antibody inhibits polymerase activity at room temperature. Activity is restored after a denaturation step in PCR cycling at 94°C, providing an automatic "hot start" in PCR and improving specificity and yield.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 100 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Contents: 4 × 1.125 mL
Composition: Each tube contains 22 U/mL complexed recombinant Taq DNA Polymerase with Platinum® Taq Antibody, 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM magnesium chloride, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, stabilizers, glycerol, and blue tracking dye.
Storage: -20°C in a non-frost-free freezer.


Manuals & protocols

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