Invitrogen™

SuperScript™ IV One-Step RT-PCR System with ezDNase™

Catalog number:  12595025

Related applications:

Reverse Transcription

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Description

The Invitrogen SuperScript IV One-Step RT-PCR System with ezDNase combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation and gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System with ezDNase include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits. Inclusion of ezDNase enzyme for use upstream of the RT step further accelerates the RT-PCR workflow through an extremely simplified genomic DNA removal step. (This one-step RT-PCR system is also available without ezDNase.)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type: RNA
Detection Method: Gel Electrophoresis
No. of Reactions: 25
Size (Final Product): 14 kb or less
Hot Start: Built-In Hot Start
PCR Method: 1-Step RT-PCR
Polymerase: Platinum™ SuperFi™ DNA Polymerase
Reverse Transcriptase: SuperScript™ IV
Shipping Condition: Dry Ice
Product Line: SuperScript™

Contents & storage

• 12.5 µL SuperScript IV RT Mix
• 625 µL 2X Platinum SuperFi RT-PCR Master Mix
• 25 µL ezDNase Enzyme
• 100 µL 10X ezDNase Buffer
• 1.25 mL nuclease-free water

Store at -20°C in a non-frost-free freezer.