Informational:Informational:Create physiologically relevant cell culture models with Gibco Human Plasma-like Medium >
Informational:Informational:Try TrypLE Express Enzyme (1X), no phenol red for free with the Aspire member program
Gibco™

TrypLE™ Express Enzyme (1X), no phenol red

Green features
Catalog number: 12604013
Gibco™

TrypLE™ Express Enzyme (1X), no phenol red

Green features
Catalog number: 12604013
Catalog Number
12604013
also known as 12604-013
Unit Size
100 mL
Price (USD)
31.63
Your Price:
Availability
***
Quantity
Catalog Number
12604021
also known as 12604-021
Unit Size
500 mL
Price (USD)
91.50
Your Price:
Availability
***
Quantity
Catalog Number
12604039
also known as 12604-039
Unit Size
20 x 100 mL
Price (USD)
488.00
Your Price:
Availability
***
Quantity
Catalog Number
12604054
also known as 12604-054
Unit Size
5 L
Price (USD)
766.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12604013
also known as 12604-013
100 mL
31.63
Your Price:
***
12604021
also known as 12604-021
500 mL
91.50
Your Price:
***
12604039
also known as 12604-039
20 x 100 mL
488.00
Your Price:
***
12604054
also known as 12604-054
5 L
766.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE™ Express is modified as follows:
WithWithout
• EDTA• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Concentration
1 X
Product Type
Cell Culture Dissociation Reagents
Sterility
Sterile-filtered
Chelators
EDTA
Enzyme Stability
Room Temperature Stable
pH Range
Check and Record
Osmolality
Check and Record
Shipping Condition
Room Temperature
Tested For
In Vitro Bioassay
Product Line
Gibco™
Form
Liquid
Phenol Red Indicator
No Phenol Red
Shelf Life
24 Months
Green Features
Energy efficient, Sustainable packaging
Quantity
100mL
Reagent Type
Trypsin Replacement
Cell Type
Mammalian
Culture Type
Adherent Cell Culture
Source
Animal origin-free
pH
6 to 8
Classification
Animal Origin-Free
State at Room Temperature
2°C to 8°C
Solution Type
Trypsin Replacement

Contents & Storage

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Figures