Get out of the cold & warm up to new Gibco BenchStable Media! Optimized for storage at room temperature »

TrypLE™ Express Enzyme (1X), no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  12604013

Related applications:

Mammalian Cell Culture

Back to top


TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE™ Express is modified as follows:
• EDTA• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.


Chelators: EDTA
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Green Features: Energy efficient, Sustainable packaging
Osmolality: Check and Record
Product Line: Gibco®
Quantity: 100mL
Shipping Condition: Room Temperature
Tested For: In Vitro Bioassay
Form: Liquid
Phenol Red Indicator: No Phenol Red
Reagent Type: Trypsin Replacement
pH Range: Check and Record
Concentration: 1 X

Contents & storage

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture