Gibco™

Advanced RPMI 1640 Medium

Green features
Catalog number: 12633012
Gibco™

Advanced RPMI 1640 Medium

Green features
Catalog number: 12633012
Catalog Number
12633012
Unit Size
500 mL
Price (USD)
Price: 42.26
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
10 x 500 mL
Price (USD)
Price: 368.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12633012500 mL
Price: 42.26
Your Price:
-
1263302010 x 500 mL
Price: 368.00
Your Price:
-
Product Overview
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Additional Information
Recommendations
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:

WithWithout
• Glucose• L-glutamine
• Non Essential Amino Acids• HEPES
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco™ Advanced RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
1X
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Serum Supplementation
Advanced (Reduced Serum)
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
No Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Quantity
500mL
With Additives
Glucose, Non Essential Amino Acids, Phenol Red, Sodium Pyruvate
Without Additives
HEPES, L-Glutamine
Glucose
D-Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
Cell Line
Sp2, Vero, Raji, Daudi, and Jurkat
Classification
Animal Origin
Solution Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations