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Gibco™
Medio Advanced RPMI 1640
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cellsMás información
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| Número de catálogo | Cantidad |
|---|---|
| 12633012 | 500 mL |
| 12633020 | 10 x 500 mL |
Número de catálogo 12633012
Precio (CLP)
50.743
Each
Cantidad:
500 mL
Precio (CLP)
50.743
Each
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.
This Advanced RPMI 1640 is manufactured as follows:
With: glucose, non-essential amino acids, sodium pyruvate, phenol red
Without: L-glutamine, HEPES
The complete formulation is available.
Gibco Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX supplement. Many cell lines do not require adaptation to this medium. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de célulasSp2, Vero, Raji, Daudi y Jurkat
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio RPMI 1640 (medio Roswell Park Memorial Institute 1640)
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónOrigen animal
FormularioLíquido
Serum LevelSuero reducido
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosGlucosa, Rojo de fenol, Piruvato sódico, Aminoácidos no esenciales
Sin aditivosSin glutamina, Sin HEPES
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación