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Gibco™

Advanced RPMI 1640 Medium

Catalog number: 12633012
Gibco™

Advanced RPMI 1640 Medium

Catalog number: 12633012
Catalog Number
12633012
Unit Size
500 mL
Price (USD)
Price: 47.57
Online price: 42.65
Your price:
Availability
-
Quantity
Catalog Number
12633020
also known as 12633-020
Unit Size
10 x 500 mL
Price (USD)
Price: 414.00
Online price: 372.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12633012500 mL
Price: 47.57
Online price: 42.65
Your price:
-
12633020
also known as 12633-020
10 x 500 mL
Price: 414.00
Online price: 372.65
Your price:
-
Product Overview
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Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.


This Advanced RPMI 1640 is manufactured as follows:
WithWithout
• Glucose• L-glutamine
• Non Essential Amino Acids• HEPES
• Sodium pyruvate 
• Phenol Red 


The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line
Sp2, Vero, Raji, Daudi, and Jurkat
Classification
Animal Origin
Concentration
1 X
Form
Liquid
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level
Reduced Serum
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives
No Glutamine, No HEPES
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

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