Advanced DMEM/F-12
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Advanced DMEM/F-12
Gibco™

Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cellsRead more
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Catalog NumberQuantity
1263402810 x 500 mL
12634010500 mL
Catalog number 12634028
Price (USD)/ Each
632.00
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Quantity:
10 x 500 mL
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Price (USD)/ Each
632.00
Add to cart
Advanced DMEM/F-12
Catalog number12634028
Price (USD)/ Each
632.00
-
Add to cart

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:
WithWithout
• Glucose• L-glutamine
• Non Essential Amino Acids• HEPES
• Sodium pyruvate 
• Phenol Red 


The complete formulation is available.

Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineMRC-5, SP2, Vero, WI-38 and Jurkat
ClassificationAnimal Origin
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum LevelReduced Serum
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 x 500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture