Sf-900™ III SFM

Catalog number:  12658001

 Related applications:

Insect Cell Culture

| Viral Vaccine Production

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Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).

cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.


Amino Acids: Non-Essential Amino Acids
Cell Line: Sf21, Sf9
Cell Type: Insect Cells
Classification: Animal Origin-Free
Concentrated: 1 X
Concentration Ratio: 1 :1
Culture Environment: Non-CO2
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Form: Liquid
Glutamine: L-Glutamine
Included Antibiotics: No antibiotics
Phenol Red Indicator: No Phenol Red
Product Line: Gibco®, Sf-900™
Product Size: 20 L
Purity or Quality Grade: Cell Culture Grade
Quantity: 1 universal bag(s)
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Sodium Pyruvate Additive: No Sodium Pyruvate
Sterility: Sterile
Volume (Metric): 20 L
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture


Manuals & protocols