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Gibco™

Fetal Bovine Serum, mesenchymal stem cell-qualified, Australia origin

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofMás información
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Número de catálogoCantidad
12664025C500 mL
Número de catálogo 12664025C
Precio (USD)
-
Cantidad:
500 mL

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Specialty
  • Specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤30 mg/dL
  • Country of Origin: Australia
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Grupo de donantesNo
Concentración de endotoxinas≤5 EU/mL
Concentración de hemoglobina≤30 mg/dL
Grado de pureza o calidadMS Cell-Qualified
Condiciones de envíoDry Ice
EspecieCattle/Bovine
Probado paraBovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria and Fungi)
AgeFetal
País de origenAustralia
FormatoBottle
Cantidad500 mL
Serum TreatmentStandard (Sterile-filtered)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.