Platinum™ Hot Start PCR Master Mix (2X)

      Platinum™ Hot Start PCR Master Mix (2X)

      Numéro de catalogue:  13000012

      Invitrogen™  Applications liées: Genotyping & Genomic Profiling | Methylation Analysis | PCR | Sanger Sequencing

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      Description

      Invitrogen™ Platinum™ Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. Platinum Hot Start PCR Master Mix retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability.

      Product features:
      • High specificity and increased yields with antibody-mediated hot-start PCR
      • Convenient room temperature reaction setup
      • Versatile formulation for broad range of amplicons

      About Platinum Taq DNA Polymerase
      Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, the Taq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).

      Using Platinum Hot Start PCR Master Mix
      Convenient Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water.Platinum Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.

      More Platinum DNA Polymerase products >
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Polymerase: Platinum™ Taq DNA Polymerase
      Hot Start: Built-In Hot Start
      Fidelity (vs. Taq): 1 X
      GC-Rich PCR Performance: Medium
      Reaction Format: SuperMix or Master Mix
      Product Overhang: 3'-A
      Reaction Speed: Standard
      Final Product Size(s): 5 kb or less
      Product Line: Platinum™
      Product Size: 50 reactions
      Shipping Condition: Dry Ice

      Contenu et stockage

      Each box contains:
      • 1.25 mL Platinum Hot Start PCR 2X Master Mix
      • 1.25 mL Platinum GC Enhancer
      • 1.25 mL water, nuclease-free

      Store at -20°C in a non-frost-free freezer.

      Documents

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